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The cells were centrifuged at 900 × g for 3 min, the supernatant was discarded, and the cell pellets were resuspended in 1 mL of KPBS supplemented with protease inhibitors (5 mg/mL aprotinin, 10 mg/mL leupeptin, and 250 mM PMSF)

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doi: 10.1016/j.ctrv.2008.03.002

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