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Other factors to consider in a pharmacokinetic study may include controls to ensure compound stability in the collected samples prior to analysis, anesthesia used prior to sacrifice and/or sample collection, and sample collection tubes (serum, plasma with anticoagulant, etc.)

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Moreover, there is no consensus on the blood vitamin B 12 lower cut-off point, which ranges from 100 to 350 pg/mL, and deficiency may occur even when the total serum vitamin B 12 is within the normal range [17,18,19,20]