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Primary antibodies were applied overnight at 4 °C, followed by HRP-conjugated secondary antibodies for 1 h at room temperature
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Key Areas of Research: Circadian: Delta-wave, REM modulation, entrainment Neuroendocrine: Corticosterone, ACTH, HPA axis Signaling: GABAergic, serotonergic, dopaminergic Neuronal: Oxidative stress, calcium dynamics, viability Together, these findings demonstrate DSIP’s multifaceted actions across circadian, endocrine, and neuronal signaling systems
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