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The apparent saturability of 125I-TNF alpha was explained by a higher nonspecific binding of monomeric than of trimeric 125I-TNF alpha to IgG

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LH/FSH dysregulation — an elevated LH-to-FSH ratio produced by abnormally rapid GnRH pulse frequency in the hypothalamus, creating a hormonal environment that stimulates androgen production while suppressing follicle maturation.[3] Chronic low-grade inflammation — elevated CRP, IL-6, and TNF-α, even in lean women with PCOS and in the absence of obesity, suggesting that inflammation is a core feature, not a secondary consequence of excess weight