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Stable isotope tracers/gas chromatography coupled to mass spectrometry (GC-MS) For the 13C 6 glucose assay, HepG2 cells were seeded at a density of 2 × 10 5 cells/incubated for a further 24 h with DMEM (Sigma, #: D5030) supplemented with 30.5 mg of 13C 6 glucose (Sigma, #: 389374) for the BG environment or 140 mg of 13C 6 glucose for the HG environment and 12C 5 glutamine (Roth, #: HN08.2), and sodium bicarbonate (Sigma, #: S5761) with 10 μM of SF or control (DMSO) without serum
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