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After each collection, supernatant was filtered through a 0.45-µm filter, and each well of MEFs received 0.5 ml of each of the corresponding retroviral supernatants (amounting to 2 ml total)

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Additionally, this research revealed an increased expression of GSH and SOD enzymes in the hippocampus of the rat brain, along with a reduction in MDA levels, thereby confirming that CU effectively mitigates the impact of oxidative stress on depression