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Each deficiency syndrome has a distinct pathophysiology: thiamine (E51) affects cellular energy metabolism
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For further differentiation into HBs, a combined protocol of BMP4, IWR-1, and TGFΞ² inhibitor SB431542 eliciting hepatic markers, including fetoprotein (AFP), albumin (ALB), and prospero homeobox 1 (PROX1) was established (Touboul et al., 2016)
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