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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4°C overnight on a rotating shaker

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Furthermore, in the presence of S-nitrosylated PDI, the formation of mutant SOD1 aggregates increases in vitro (Jeon et al., 2014)

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