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Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

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Here's how: It increases slow-wave (deep) sleep The most restorative stage of your sleep cycle is known as slow-wave sleep (SWS), also referred to as deep sleep