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To generate various mGFPmut2-PIF4 recombinant proteins, the coding sequence (CDS) of mGFPmut2 , the GS-linker (GAPGSAGSAAGGSG), and the wild-type or mutant PIF4 were cloned into pET28a vectors between EcoRI and XhoI

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[PubMed] [Google Scholar] Chedid A, Mendenhall CL, Tosch T, et al