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40–50 µg of plasmid DNA was mixed with 200 µl of 2.5 M CaCl 2 and 1.7 ml H 2 O, after which 2 ml of 2xHBS buffer (50 mM HEPES pH 7.05, 140 mM NaCl, 1.5 mM Na 2 HPO 4 ×2H 2 O) was added dropwise while vortexing or bubbling the solution to enable formation of precipitates

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[DOI] [PubMed] [Google Scholar] 27.Blagojevic-Bucknall M., Mallen C., Muller S., Hayward R., West S., Choi H., Roddy E