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Chronic inflammation disrupts endothelial integrity and reduces NO bioavailability, while inflammasomes like NOD-like receptor thermal protein domain associated protein 3 may activate oxidative stress, and finally aggravate vascular damage [70]
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Cells were treated with ENDOs or vehicle and TLR9 activation in response to CpG (5 µM) was analyzed by measuring SEAP activity, monitored using QUANTI-Blue (InvivoGene) following the manufacturer’s recommendations
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