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Precise control of operational parameters—such as power, temperature, and extraction duration—is essential, as excessive energy input or prolonged treatment can degrade sensitive polyphenolic structures, potentially lowering yield or altering compound profiles (52)

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For the cross-sectional study, plasma was removed and deproteinised with 6 % sulfosalicylic acid (1:1) to remove plasma proteins and enable reliable determination of free plasma CYS (Reference Tôrres, Miller and Rogers32)