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Following this blocking step, they were incubated overnight at 4 °C with rat monoclonal anti-pectin antibodies (Plant Probes) either JIM7 (recognizing partially methylesterified homogalacturonan) or LM19 (recognizing partially and fully de-esterified homogalacturonan) diluted 1:50 in 0.2% BSA/PBS pH 7.2