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To permeabilize the cuticle, the worms were incubated for 1 h at room temperature with gentle rocking in 1× Modified Ruvkun’s Witches Brew buffer (diluted from a 2× stock: 3.7% formaldehyde, 160 mM KCl, 40 mM NaCl, 14 mM EDTA, 0.2% βME)