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In a 6 multi-well plate, 1.5 × 10 5 cells were plated and transfected with 900 ng of pHIV-dTomato and 100 ng of pIRES2-EGFP (Clontech #6029-1, Mountain View, CA, USA), a transfection efficiency control, using Lipofectamine 3000 Transfection Reagent (Invitrogen, Life Technologies)

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Size and Gauge:30 Gauge, 0.5 mL, 1/2-inch

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