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If you add 4ml to maintain the 2.5mg/ml concentration, you have twice the total volume to work through

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TrkB, belonging to the Trk family of tyrosine kinase receptors, is a high-affinity specific receptor for BDNF and one of the most widely distributed neurotrophic receptors in the brain, which is highly enriched in the cerebral cortex, hippocampus, striatum, and brainstem [38]