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2.3 Laboratory-scale glutathione affinity chromatography Unless otherwise indicated, laboratory-scale GSH affinity chromatography was performed with GSTPrep FF 16/10 columns packed with GSH Sepharose 4 FF resin (Cat#: 28936550, Cytiva) run at a 6 min residence time using an AKTA Pure 150 M FPLC system (Cytiva) with UNICORN system control software (Cytiva)

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doi: 10.1016/j.cell.2007.10.004